Nanosatellite-substrate complex and method of regulating macrophage adhesion and polarization using the same

A nanosatellite-substrate complex for regulating macrophage adhesion and polarization comprising:

• a substrate;
• a core-shell-type magnetic nanoparticle disposed to be spaced apart from a first side of the substrate;
• a plurality of gold nanoparticles connected to one side of the magnetic nanoparticle;
• a plurality of first linkers connecting between the substrate and the plurality of gold nanoparticles;
• a plurality of second linkers connecting between the plurality of gold nanoparticles and a plurality of ligands;
• and
• the plurality of ligands connected to the plurality of gold nanoparticles via the plurality of second linkers,
• wherein the plurality of gold nanoparticles is are connected to the plurality of ligands via the plurality of second linkers to form a nanoassembly,
• the magnetic nanoparticle is conjugated to the nanoassembly to form a nanosatellite structure,
• the nanoassembly comprises the plurality of gold nanoparticles, the plurality of second linkers connected to the plurality of gold nanoparticles, and the plurality of ligands connected to the plurality of second linkers, and
• a plurality of nanoassemblies are comprised in the nanosatellite structure, and
• wherein the plurality of the nanoassemblies are disposed adjacent to each other, and a distance between the plurality of gold nanoparticles in the plurality of nanoassemblies disposed adjacent to each other includes at least one of a first distance, a second distance and a third distance,
• wherein the first distance is 2 nm to 4 nm, the second distance is 15 nm to 20 nm, and the third distance is 18 nm to 22 nm.