Antibacterial agents and methods
AssigneeFolium Food Science Limited
InventorsMartin Woodward, Tristan Cogan, Edward Fuchs, Holger Kneuper, Alessandro Riccio, Christian Grøndahl, Jasper Clube
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Abstract
The invention relates to means for carrying out conjugation between bacteria, and in particular the invention relates to carrier bacteria comprising antimicrobial agents and methods of use. The carrier bacteria are capable of conjugative transfer of DNA encoding the agent to a target cells. The invention further relates to growth or feed conversion ratio promotion in animals. The invention further relates to killing Salmonella or inhibiting the growth or proliferation of Salmonella.
Claims12
A method for enhancing feed conversion rate in a chicken, wherein the method comprises:
- (a) orally administering to the chicken a plurality of carrier cells, wherein the carrier cells are E. coli S17,
- wherein the chicken comprises target bacterial cells,
- wherein the target bacterial cells comprise Salmonella enterica cells,
- wherein each carrier cell is a bacterial cell comprising a first episomal DNA encoding an antibacterial agent,
- wherein the antibacterial agent comprises a guide RNA or crRNA and a Cas nuclease of a CRISPR/Cas system, and
- wherein the first DNA is transferred from the carrier cells into the target cells by conjugative transfer of the first DNA into the target cell for expression therein of the agent;
- and
- (b) performing husbandry of the chicken, thereby killing target cells in the chicken and enhancing feed conversion rate of the chicken as compared to a chicken not administered with the plurality of bacterial carrier cells and having the target bacterial cells.
Dependencies are inferred from the claim text.