Vesicle and use thereof
AssigneeEv Cell Biotech (Guangzhou) Co., Ltd.
InventorsXiao Zhang, Xiaoxing Kou, Songtao Shi
Life of this patent
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Abstract
Provided are a vesicle and the use thereof. The vesicle is an induced vesicle, and the sources thereof include stem cells or somatic cells, and the possessed markers include Syntaxin 4. Compared with an exosome in mesenchymal stem cells, the vesicle can specifically express Syntaxin 4 and can be used to distinguish characteristic markers of MSC-derived vesicles and exosomes. The vesicle can play a procoagulant effect in vitro, can improve the bleeding tendency of mice with hemophilia after in vivo injection, and can be used for the treatment of improving the bleeding tendency of hemophilia. In addition, the vesicle can be expelled through the skin and hair.
Claims16
A method for detecting presence of an induced apoptotic mesenchymal stem cell vesicle in a sample, comprising:
- detecting Syntaxin 4, Annexin V, Flotillin-1, Cadherin 11, or Integrin alpha 5 in the sample;
- and
- determining whether:
- an expression level of the Annexin V in the sample is more than 1 to 2 fold of an expression level of Annexin V in an exosome, a migrant, a microbubble, or an ectosome;
- an expression level of the Flotillin-1 in the sample is 2 to 3 fold of an expression level of Flotillin-1 in the exosome, the migrant, the microbubble, or the ectosome;
- an expression level of the Cadherin 11 in the sample is more than 1 to 3 fold of an expression level of Cadherin 11 in the exosome, the migrant, the microbubble, or the ectosome;
- an expression level of the Integrin alpha 5 in the sample is 3 to 4 fold of higher than an expression level of Integrin alpha 5 in the exosome, the migrant, the microbubble, or the ectosome;
- or
- an expression level of the Syntaxin 4 in the sample is 2 to 6 fold of higher than an expression level of Syntaxin 4 in the exosome, the migrant, the microbubble, or the ectosome.
Dependencies are inferred from the claim text.