# US 12,758,518 B2 · Production of omega-3 long chain polyunsaturated fatty acids > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,758,518 B2 - Document ID: US12758518B2 - Status: Granted - Kind: B2 - Application number: 17/139,232 - Filed: 2020-12-31 - Published: 2021-09-23 - Granted: 2026-10-13 - Earliest claimed: 2012-04-12 - Assignees: Rothamsted Research Limited - Applicants: Rothamsted Research Ltd. - Inventors: Johnathan Napier; Olga Sayanova; Noemi Ruiz Lopez; Richard Haslam - Web page: https://patentgrep.com/patent/US12758518B2 ## CPC - A61K 31/202: having three or more double bonds, e.g. linolenic (eicosanoids, e.g. leukotrienes A61K31/557) - C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A recombinant camelina plant or cell comprising one or more polynucleotides encoding a A6-desaturase, a A6-elongase and a A5-desaturase operably linked with one or more regulatory sequences. ## Claims ### Claim 1 An oil extracted from genetically modified Brassicaceae seeds, wherein said oil comprises (a) eicosapentaenoic acid (EPA) wherein EPA constitutes at least 15% (mol %) of the total fatty acid content of said oil and (b) γ-linolenic acid (GLA), wherein GLA constitutes less than 10% (mol %) of the total fatty acid content of said oil, wherein said oil is directly obtained by pressing or extracting it from the seed of said genetically modified Brassicaceae, and wherein said genetically modified Brassicaceae seeds comprise exogenous polynucleotides encoding an acyl-CoA-dependent Δ6-desaturase, a Δ6-elongase, and a Δ5-desaturase, wherein each polynucleotide is operably linked to one or more promoters that direct expression of said polynucleotides during development of the seed, wherein the Δ6-desaturase comprises an amino acid sequence that has at least 95% sequence identity to SEQ ID NO: 2 or 20, and wherein said Brassicaceae is from the Camelina genus. ### Claim 2 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds according to claim 1, wherein the EPA constitutes at least 20% (mol %) of the total fatty acid content of said oil. ### Claim 3 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds according to claim 1, wherein the GLA constitutes less than 7% (mol %) of the total fatty acid content of said oil. ### Claim 4 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds according to claim 1, wherein the oil comprises phosphatidylcholine. ### Claim 5 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds according to claim 1, wherein the oil comprises one or more phosphatidylethanolamine species wherein the total number of carbon atoms:double bonds of the fatty acid acyl groups of said phosphatidylethanolamine species is selected from the group consisting of 34:4, 36:7, 38:8, 38:7, 38:6, 38:5, 40:10, 40:9, 40:8, 40:7, 40:6, 40:5. ### Claim 6 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds according to claim 1, wherein the seed oil comprises triglycerides wherein the number of carbon atoms:double bonds of said triglycerides is selected from the group consisting of 58:8, 58:9 and 58:10. ### Claim 7 (depends on 1) A feedstuff, food, cosmetic or pharmaceutical comprising the oil according to claim 1. ### Claim 8 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds of claim 1, wherein the recombinant Brassicaceae plant further comprises one or more polynucleotides encoding at least one of a Δ12-desaturase and/or an ω3-desaturase. ### Claim 9 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds of claim 1, wherein the Δ6-desaturase is derived from Ostreococcus tauri or Ostreococcus RCC809, the Δ6-elongase is derived from Physcomitrella patens and the Δ5-desaturase is derived from Thraustochytrium sp. ### Claim 10 (depends on 1) The oil extracted from genetically modified Brassicaceae seeds of claim 1, wherein the Δ6-elongase comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 4, SEQ ID NO: 22 or SEQ ID NO: 24, and the Δ5-desaturase comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 6 or SEQ ID NO: 10. ### Claim 11 An oil extracted from genetically modified Brassicaceae seeds, wherein said oil comprises (a) eicosapentaenoic acid (EPA) wherein EPA constitutes at least 15% (mol %) of the total fatty acid content of said oil and/or docosahexaenoic acid (DHA) wherein DHA constitutes at least 5% (mol %) of the total fatty acid content of said oil and (b) γ-linolenic acid (GLA), wherein GLA constitutes less than 10% (mol %) of the total fatty acid content of said oil, wherein said oil is directly obtained by pressing or extracting it from the seed of said genetically modified Brassicaceae, and wherein said genetically modified Brassicaceae seeds comprise exogenous polynucleotides encoding an acyl-CoA-dependent Δ6-desaturase, a Δ6-elongase, a Δ5-desaturase, a Δ5-elongase and a Δ4-desaturase, wherein each polynucleotide is operably linked to one or more promoters that direct expression of said polynucleotides during development of the seed, wherein the Δ6-desaturase comprises an amino acid sequence that has at least 95% sequence identity to SEQ ID NO: 2 or 20, and wherein said Brassicaceae is from the Camelina genus. ### Claim 12 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds according to claim 11, wherein the EPA constitutes at least 20% (mol %) of the total fatty acid content of said oil. ### Claim 13 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds according to claim 11, wherein the DHA constitutes at least 13% (mol %) of the total fatty acid content of said oil. ### Claim 14 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds according to claim 11, wherein the oil comprises EPA and DHA, wherein the EPA and DHA constitutes at least 20% of the total fatty acid content of said oil. ### Claim 15 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds according to claim 11, wherein the GLA constitutes less than 7% (mol %) of the total fatty acid content of said oil. ### Claim 16 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds of claim 11, wherein the Δ6-desaturase is derived from Ostreococcus tauri or Ostreococcus RCC809, the Δ6-elongase is derived from Physcomitrella patens and the Δ5-desaturase is derived from Thraustochytrium sp. ### Claim 17 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds of claim 11, wherein the Δ6-elongase comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 4, SEQ ID NO: 22 or SEQ ID NO: 24, and the Δ5-desaturase comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 6 or SEQ ID NO: 10, the Δ5-elongase comprises an amino acid sequence having at least 95% identity to SEQ ID NO:8, and the Δ4-desaturase comprises an amino acid sequence having at least 95% identity to SEQ ID NO:16, SEQ ID NO: 18 or SEQ ID NO:26. ### Claim 18 (depends on 11) The oil extracted from genetically modified Brassicaceae seeds of claim 11, wherein the recombinant Brassicaceae plant further comprises one or more polynucleotides encoding at least one of a Δ12-desaturase or an ω3-desaturase.