Method for preparing drug-loaded silk fibroin nanocapsule, and product thereof

A hollow silk fibroin nanocapsule used for drug-loading, characterized in that the hollow silk fibroin nanocapsule is prepared by a method comprising:

• performing a coating treatment of applying alternating layers of silk fibroin and 3-aminopropyl triethoxysilane on functionally modified polystyrene spheres having a diameter of 0.05-0.1 μm, to ultimately obtain a multi-layer coated polystyrene composite with a negative or positive surface potential, and finally removing the functionally modified polystyrene spheres with a template-removing reagent, to obtain a hollow silk fibroin nanocapsule,
• wherein the 3-aminopropyl triethoxysilane is a cross-linking agent to bind and induce silk fibroin to be coated alternatively, thereby forming layer-by-layer assembly,
• wherein, when the functionally modified polystyrene spheres are positive potential, silk fibroin is used for a first coating, and in the case of negative potential spheres, 3-aminopropyl triethoxysilane is used for the first treatment,
• wherein the functionally modified polystyrene spheres comprise an amination surface modification for positive potential or a carboxylation surface modification for negative potential, and
• wherein when loading a molecule drug with positive potential, a hollow silk fibroin nanocapsule characterized with negative surface potential from the final coating treatment with silk fibroin is used, when loading a negative potential molecule drug, a hollow silk fibroin nanocapsule characterized with positive surface potential from the final coating treatment with 3-aminopropyl triethoxysilane is used.