Methods and compositions for dendritic cell targeting nano-delivery
AssigneeRock Biomedical, Inc.
InventorsChi-Huey Wong, Jeng Shin Lee, Chen-Yo Fan, Szu-Wen Wang, Chung-Yi Wu
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Abstract
The present disclosure relates to novel compounds, methods, and cell-targeting formulations, e.g., a lipid nanoparticle (LNP) for targeted delivery to a tissue or a cell type. The compound and formulation provided herein are designed to have a targeting moiety configured to provide selective delivery features for the formulation and a lipid tail for being incorporated into the bilayer membrane of the formed lipid nanoparticle.
Claims35
A lipid nanoparticle, comprising a membrane defining an inner space, wherein the membrane is formed with a plurality of lipid components, wherein the plurality of lipid components comprises a compound comprising Formula 1 or Formula 2:
- wherein R1 comprises a formula of R2-RA—, wherein RA is an attachment group and R2 is a substituted or non-substituted glycosyl group, and wherein the attachment group comprises an aryl and an alkylamide, having a structure of -aryl-alkyl-NH—C(O)—, and the aryl moiety attaches to R2;
- wherein X1 and X2 are each independently hydrogen, C1-30 alkyl, C1-30 alkenyl, C1-30 alkynyl, aryl, aryloxy, or a substituted version thereof, or
- —(CH2)nX4, n is 0 to 30, and X4 is hydrogen, aryl, aryloxy, heterocyclic group, or a substituted version thereof, provided that when X4 is a heterocyclic group, the heterocyclic group comprises 1 to 3 heteroatoms, selected from the group consisting of O, S, and N, or a combination thereof;
- and
- wherein X3 is hydrogen, C1-6 alkyl, or hydroxyl.
A kit for preparing a lipid nanoparticle, comprising:
- a first reagent, comprising a compound having Formula 1 or Formula 2:
- wherein R1 comprises a formula of R2-RA—, wherein RA is an attachment group and R2 is a substituted or non-substituted glycosyl group, and wherein the attachment group comprises an aryl and an alkylamide, having a structure of -aryl-alkyl-NH—C(O)—, and the aryl moiety attaches to R2;
- wherein X1 and X2 are each independently hydrogen, C1-30 alkyl, C1-30 alkenyl, C1-30 alkynyl, aryl, aryloxy, or a substituted version thereof, or
- —(CH2)nX4, n is 0 to 30, and X4 is hydrogen, aryl, aryloxy, heterocyclic group, or a substituted version thereof, provided that when X4 is a heterocyclic group, the heterocyclic group comprises 1 to 3 heteroatoms, selected from the group consisting of O, S, and N, or a combination thereof, and
- wherein X3 is hydrogen, C1-6 alkyl, or hydroxyl;
- and
- a second reagent, comprising an ionizable lipid, a helper lipid, or a mixture thereof.
Dependencies are inferred from the claim text.