Nucleic acid containing nanoparticles
AssigneeBio-TRIP B.V.
InventorsRoy Van Der Meel, Willem J. M. Mulder, Ewelina Kluza, Stijn Hofstraat, Tom Anbergen, Robby Cornelis Zwolsman, Henricus Marie Janssen, Pieter Michele Fransen
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Abstract
Herein disclosed are nanoparticles comprising a phospholipid, apolipoprotein and/or an apolipoprotein mimetic, sterol, cationic lipid or ionizable cationic lipid and a nucleic acid and compositions comprising such nanoparticles and a method for preparing such nanoparticles. The nanoparticles may be used as a medicament, such as in the treatment of a disease by stimulating or inhibiting an innate immune response.
Claims38
A nanoparticle comprising a core surrounded by a surface layer, wherein:
- the core comprises a nucleic acid and a cationic or ionizable cationic lipid;
- and
- the surface layer is a monolayer comprising:
- a phospholipid,
- a sterol, and
- an apolipoprotein or an apolipoprotein mimetic or a combination thereof.
A method for producing a nanoparticle, comprising the step of:
- a) rapid mixing of lipid components in organic solvent with a nucleic acid in an aqueous buffer to produce lipid nanoparticles, wherein the lipid components comprise a phospholipid, a sterol, a cationic lipid or ionizable cationic lipid, wherein the aqueous buffer has a pH of 5.0 or lower;
- and
- b) rapid mixing of the lipid nanoparticles with an apolipoprotein, an apolipoprotein mimetic, or a combination thereof to produce the nanoparticle at a pH between 6.0 and 8.0.
Dependencies are inferred from the claim text.