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US 12,757,915 B2Granted
Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
AssigneeNovozymes A/S
InventorsKurt Creamer, Kalpesh Parekh, James Lavigne
Life of this patent
Estimating the term…
Abstract
The present invention relates to a process for producing a high protein feed ingredient from a whole stillage byproduct produced in a starch-containing grain dry milling process for producing a fermentation product, as well as enzyme blends used in the processes for partitioning a greater amount of protein from the whole stillage byproduct into the high protein fraction, rather than being retained in the wet cake, to produce a high protein feed ingredient.
Claims38
A method for producing a feed ingredient from whole stillage, the method comprising:
- a) performing a starch-containing grain dry milling process for producing a fermentation product to produce a fermentation product and a whole stillage, wherein step a) comprises:
- (i) saccharifying the starch-containing grain at a temperature below the initial gelatinization temperature with an alpha-amylase and a glucoamylase;
- and
- (ii) fermenting using a fermenting organism to produce the fermentation product,
- wherein an enzyme blend comprising at least two hemicellulases and at least two beta-glucanases and a cellulolytic composition is added to whole stillage,
- wherein the hemicellulases comprise a GH10 xylanase and a GH62 α-L-arabinofuranosidase,
- wherein the GH10 xylanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 20 to 397 of SEQ ID NO:
- 2,
- wherein the GH62 α-L-arabinofuranosidase has an amino acid sequence comprising at least 85% sequence identity to amino acids 17 to 325 of SEQ ID NO:
- 11;
- wherein the beta-glucanases are from a family selected from the group consisting of GH5_15 and GH64,
- wherein the GH5_15 family beta-glucanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 17 to 408 of SEQ ID NO:
- 15,
- wherein the GH64 family beta-glucanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 17 to 447 or 64 to 447 of SEQ ID NO:
- 20;
- wherein the cellulolytic composition comprises:
- (i) a cellobiohydrolase I having an amino acid sequence comprising at least 85% sequence identity to amino acids 27 to 532 of SEQ ID NO:
- 21;
- (ii) a beta-glucosidase having an amino acid sequence comprising at least 85% sequence identity to amino acids 20 to 863 of SEQ ID NO:
- 23 with the substitutions F100D, S283G, N456E, and F512Y;
- and
- (iii) an endoglucanase I having an amino acid sequence comprising at least 85% sequence identity to amino acids 23 to 459 of SEQ ID NO:
- 44;
- b) separating the whole stillage into an insoluble solids portion and a thin stillage portion;
- c) separating the thin stillage portion into at least a first separated water-soluble solids portion, and at least a first separated protein portion;
- d) optionally separating at least the first separated protein portion into at least a second separated water-soluble solids portion, and at least a second separated protein portion;
- and
- e) drying at least the first separated protein portion, and/or optionally at least the second separated protein portion, to define a protein product, wherein the protein product is a feed ingredient comprising at least 40 wt percent protein on a dry basis.
25 more elements
A method for producing a feed ingredient from whole stillage, the method comprising:
- a) performing a starch-containing grain dry milling process for producing a fermentation product to produce a fermentation product and a whole stillage, wherein step a) comprises:
- (i) liquefying the starch-containing grain with an alpha-amylase;
- (ii) saccharifying the liquefied material obtained in step (i) with a glucoamylase;
- and
- (iii) fermenting using a fermenting organism to produce the fermentation product,
- wherein an enzyme blend comprising at least two hemicellulases and at least two beta-glucanases and a cellulolytic composition is added to whole stillage,
- wherein the hemicellulases comprise a GH10 xylanase and a GH62 α-L-arabinofuranosidase,
- wherein the GH10 xylanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 20 to 397 of SEQ ID NO:
- 2,
- wherein the GH62 α-L-arabinofuranosidase has an amino acid sequence comprising at least 99% sequence identity to amino acids 17 to 325 of SEQ ID NO:
- 11;
- wherein the beta-glucanases are from a family selected from the group consisting of GH5_15 and GH64,
- wherein the GH5_15 family beta-glucanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 17 to 408 of SEQ ID NO:
- 15,
- wherein the GH64 family beta-glucanase has an amino acid sequence comprising at least 85% sequence identity to amino acids 17 to 447 or 64 to 447 of SEQ ID NO:
- 20;
- wherein the cellulolytic composition comprises:
- (i) a cellobiohydrolase I having an amino acid sequence comprising at least 85% sequence identity to amino acids 27 to 532 of SEQ ID NO:
- 21;
- (ii) a beta-glucosidase having an amino acid sequence comprising at least 85% sequence identity to amino acids 20 to 863 of SEQ ID NO:
- 23 with the substitutions F100D, S283G, N456E, and F512Y;
- and
- (iii) an endoglucanase I having an amino acid sequence comprising at least 85% sequence identity to amino acids 23 to 459 of SEQ ID NO:
- 44;
- b) separating the whole stillage into an insoluble solids portion and a thin stillage portion;
- c) separating the thin stillage portion into at least a first separated water-soluble solids portion, and at least a first separated protein portion;
- d) optionally separating at least the first separated protein portion into at least a second separated water-soluble solids portion, and at least a second separated protein portion;
- and
- e) drying at least the first separated protein portion, and/or optionally at least the second separated protein portion, to define a protein product, wherein the protein product is a feed ingredient comprising at least 40 wt percent protein on a dry basis.
26 more elements
Dependencies are inferred from the claim text.