Sesame plants resistant to acetolactate synthase-inhibiting herbicides, compositions and methods for producing same
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsZvi Peleg, Yaron Gadri, Asaf Avneri
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Abstract
The present invention relates to sesame plants resistant to herbicides that inhibit the plant enzyme acetolactate synthase (ALS), and further to compositions and methods for producing the same.
Claims17
A sesame (Sesamum indicum L.) plant or a part thereof comprising at least one cell comprising a mutant acetolactate synthase encoding polynucleotide (mALS), wherein the mALS encodes a mutant acetolactate synthase (mALS) protein having a reduced affinity to at least one acetolactate synthase (ALS)-inhibiting herbicide compared to a wild-type S. indicum ALS (SiALS) protein, wherein the mALS protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO:
- 1 and comprising the amino acid valine at position 188, and wherein the plant is resistant to at least one ALS-inhibiting herbicide.
A method for producing a sesame plant having tolerance and/or resistance to at least one ALS-inhibiting herbicide, the method comprising introducing at least one mutation in at least one allele of the plant endogenous ALS encoding gene, wherein the at least one mutation results in an encoded ALS protein comprising an amino acids sequence having at least 95% identity to the amino acid sequence set forth in SEQ ID NO:
- 1 and comprising the amino acid valine at position 188, characterized by a reduced affinity to at least one ALS-inhibiting herbicide compared to an ALS protein encoded by a non-mutated gene, thereby producing the sesame plant having tolerance and/or resistance to at least one ALS-inhibiting herbicide.
An isolated polynucleotide encoding acetolactate synthase (ALS) protein having a reduced affinity to at least one ALS-inhibiting herbicide compared to a wild-type S. indicum ALS protein, wherein the encoded ALS protein has at least 95% identity to the amino acid sequence set forth in SEQ ID NO:1 and comprising the amino acid valine at position 188.
A method for identifying a sesame plant having an enhanced tolerance and/or resistance to at least one type of ALS-inhibiting herbicide, the method comprising detecting, in a genetic material obtained from the plant, the presence of a nucleic acid marker amplified by a pair of primers comprising the nucleic acid sequence set forth in SEQ ID NO:5 and SEQ ID NO:6.
Dependencies are inferred from the claim text.