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US 12,754,163 B2Granted
Integrated method for urinary prostate specific Antigen N-glycosylation profiling by capillary electrophoresis
AssigneePannon Egyetem
InventorsGábor Járvás, Balázs Reider, Hajnalka Jankovics, Ferenc Vonderviszt, András Guttman
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Abstract
In the present invention a method is provided for capillary electrophoresis-based (CE-based) comprehensive N-glycan analysis of urinary PSA. The method is useful for analysis of PSA glycans from urine and for diagnosis of PSA-related conditions.
Claims12
A method for PSA-analysis from urine sample of a male mammal, wherein said method comprises the steps of
- providing urine sample,
- preparing said urine sample for analysis,
- separating PSA from said urine sample by affinity separation using PSA-binding molecules to obtain enriched PSA,
- releasing glycans from the enriched PSA to obtain released PSA-glycans, labeling the released PSA glycans by a fluorescent dye, to obtain fluorophore labeled released PSA glycans,
- analyzing the released labeled glycans by separation with capillary electrophoresis (CE) wherein detection of the separated, fluorophore labeled, released PSA glycans is carried out by fluorescent detection.
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Dependencies are inferred from the claim text.