Isolation and analysis of terpenes
AssigneeOrange Photonics, Inc.
InventorDylan Elmer Wilks
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Abstract
In accordance with embodiments of the present invention, a terpene-rich sample is prepared for terpene analysis using liquid chromatography via an extraction method that takes little time, uses minimal external equipment, and permits direct injection of extracted terpenes into a liquid chromatography instrument for analysis. An embodiment of the invention involves preparing a terpene-containing sample for analysis by liquid chromatography by liquid extraction; heating the liquid extract in a vial that contains a filter medium or solvent; collecting the terpenes in the medium by the vapor pressure forced through the filter from heating; and eluting the collected terpenes into a vial or directly into a chromatography injector.
Claims16
A method of analyzing terpenes in a sample, the method comprising the steps of:
- a. weighing and preparing the sample for extraction;
- b. adding the sample and an extraction solvent to a container having an internal volume ranging from 1 to 50 mL;
- c. agitating the container;
- d. heating the sample to force the extraction solvent and any extracted terpenes through a collection filter having therein a medium that binds terpenes;
- and
- e. eluting bound terpenes from the medium and injecting the terpenes into a chromatography instrument in a single step for analysis.
A vial assembly for collection and analysis of terpenes from a sample, the vial assembly comprising:
- a. a heat-resistant container portion configured to contain the sample therewithin, the container portion being open at one end thereof and having an internal volume ranging from 1 to 50 mL;
- and
- b. a cap configured to sealably cover and fasten to the open end of the container portion to thereby fixedly suspend a medium over the container portion and the sample therewithin and comprising, in an interior portion thereof, the medium that binds to volatilized terpenes, and a connector for establishing fluid communication between the medium and an analytical instrument.
Dependencies are inferred from the claim text.