Systems and methods for meso-dissection
AssigneeRoche Molecular Systems, Inc.
InventorsMichael Barnes, Christophe Chefd'hotel, Srinivas Chukka, Mohammad Qadri
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Abstract
The subject disclosure presents systems and methods for improved meso-dissection of biological specimens and tissue slides including importing one or more reference slides with annotations, using inter-marker registration algorithms to automatically map the annotations to an image of a milling slide, and dissecting the annotated tissue from the selected regions in the milling slide for analysis, while concurrently tracking the data and analysis using unique identifiers such as bar codes.
Claims21
An instrument for meso-dissection, comprising:
- a processor;
- and
- a memory coupled to the processor, the memory to store computer-readable instructions that, when executed by the processor, cause the processor to perform operations comprising:
- registering one or more annotations derived from one or more reference images onto a milling image, wherein the milling image is derived from a milling specimen disposed on a milling slide, wherein at least one of the one or more reference images are stained for the presence of one or more biomarkers and/or stained with hematoxylin and/or eosin, and wherein the one or more reference images and the milling image are derived from one or more serial sections of a biological specimen;
- and
- dissecting one or more portions of the milling specimen disposed on the milling slide based on the annotations registered to the milling image,
- wherein the milling specimen is stained differently than the one or more reference images.
A tangible non-transitory computer-readable medium to store computer-readable code that is executed by a processor to perform operations comprising:
- importing a plurality of annotated reference images, wherein each annotated reference image of the plurality of annotated reference images includes one or more reference annotations;
- selecting, by a user interface, a permutation or combination of the one or more reference annotations from at least one of the plurality of annotated reference images;
- generating a milling annotation for milling a tissue specimen based on the selected permutation or combination of reference annotations;
- and milling the tissue specimen using the generated milling annotation, wherein the milling annotation is generated based on automatic registration of an annotation generated on an external whole-slide scanner on to a live capture of the tissue specimen.
A tangible non-transitory computer-readable medium to store computer-readable code that is executed by a processor to perform operations comprising:
- automatically registering a plurality of reference annotations with a live-captured image of a tissue sample to be dissected, wherein the plurality of reference annotations are imported from a whole-slide scanner;
- generating a milling annotation for dissection of the tissue sample to be dissected based on the results of the automatic registration;
- determining, based on the generated milling annotation, one or more milling metrics;
- and dissecting the tissue sample based on the determined one or more milling metrics.
Dependencies are inferred from the claim text.