Method of producing allolactose
AssigneeChr. Hansen A/S
InventorsVojislav Vojinovic, Kim Ib Soerensen, Laurent Guinot
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Abstract
The invention relates to a method of obtaining a composition comprising allolactose by a applying one or more glucose-deficient lactic acid bacteria strains. The invention also relates to the use of said strains for the preparation of a food product comprising allolactose and to the use of said strains for increasing the content of allolactose in a food product. The invention also relates to a food product comprising allolactose and one or more glucose-deficient lactic acid bacteria strains.
Claims12
A method for producing a composition comprising allolactose, said method comprising:
- (a) inoculating a substrate comprising lactose with one or more glucose-deficient lactic acid bacteria strains, wherein said one or more glucose-deficient lactic acid bacteria strains have a mutation in a glcK gene encoding a glucokinase protein thereby causing the glucose deficiency;
- and
- (b) fermenting said inoculated substrate to obtain a composition comprising allolactose;
- wherein at least one of the one or more glucose-deficient lactic acid bacteria strains is selected from a group consisting of Streptococcus thermophilus strains deposited at DSMZ under accession number DSM 33719, DSM 33762, and a mutant strain derived from either thereof, wherein the mutant strain is obtained by using one of the deposited strains as starting material, and wherein the mutant has retained or further improved the lactose fermenting property and/or the glucose secreting property of said deposited strain.
A composition comprising at least 0.04% w/w allolactose, wherein said composition further comprises one or more glucose-deficient lactic acid bacteria strains, wherein said one or more glucose-deficient strains have a mutation in a glcKL gene encoding a glucokinase protein causing the glucose deficiency, wherein at least one of the one or more glucose-deficient lactic acid bacteria strains is selected from a group consisting of Streptococcus thermophilus strains deposited at DSMZ under accession number DSM 33719, DSM 33762, and a mutant strain derived from either thereof, wherein the mutant strain is obtained by using one of the deposited strains as starting material, and wherein the mutant has retained or further improved the lactose fermenting property and/or the glucose secreting property of said deposited strain.
Dependencies are inferred from the claim text.