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US 12,753,422 B2Granted
Method for preparing natural nitrite
AssigneeCj Cheiljedang Corporation
InventorsJin Hee Han, Sung Hoon Jo, Hyo Jung Yoon
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Abstract
The present application relates to a method of producing natural nitrite, the method including preparing an extract from a plant containing nitrate, activating nitrate reductase within the plant extract, and inactivating the nitrate reductase. According to the method of producing the natural nitrite, nitrite may be produced in a high concentration from natural raw materials without inoculation of reducing bacteria.
Claims7
A method of producing natural nitrite, comprising:
- preparing an extract from a plant containing nitrate;
- activating endogenous nitrate reductase within the plant extract;
- and
- inactivating the nitrate reductase,
- wherein the activating of the nitrate reductase comprises adding the plant extract into a phosphate buffer solution or a carbonate buffer solution and maintaining the pH of the buffer solution containing the plant extract within a range of pH 6 to pH 9 at a temperature of 20 to 40° C.,
- wherein the inactivating of the nitrate reductase comprises heating the buffer solution containing the plant extract to a temperature of 60° C. or higher, and
- wherein the plant comprises nitrate in a concentration of 3,000 ppm or more.
4 more elements
Dependencies are inferred from the claim text.