Vector for co-expressing genes of interest
AssigneeGat Biosciences, S.L.
InventorsOlga Durany Turk, Jordi Segura De Yebra, Jaume Mercade Roca, Maria Teresa Lopez Cerro, Cristina Lopez Paz, Lourdes Tatiana Roque Navarro
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Abstract
An expression vector for the co-expression of different polynucleotides in microalgal cells and to the expression cassettes having both polynucleotides is provided. Also provided are a host cell, a method for expressing the proteins of interest and a method for selecting a cell co-expressing two proteins of interest in a stoichiometric manner.
Claims14
An expression vector for the co-expression of a first and second polynucleotides of interest in microalgal cells, wherein said first and second polynucleotides of interest are provided respectively in a first and second expression cassettes, each expression cassette comprising a 5′ cis regulatory region and a 3′ cis regulatory region operatively linked with the first and second polynucleotides,
- wherein the 5′ and 3′ cis regulatory regions in the first expression cassette comprise, respectively, an RPL23 gene promoter and a 3′UTR of the RPL23 gene, and the 5′ and 3′ cis regulatory regions of the second expression cassette comprise respectively an FDX gene promoter and a 3′UTR of the FDX gene,
- wherein the expression vector further comprises a third expression cassette comprising a HSP70A/RBCS2 chimeric promoter and a 3′UTR of the RBCS2 gene as cis regulatory regions, and
- wherein the first and second polynucleotide encode two different subunits of a heterodimeric protein.
Dependencies are inferred from the claim text.