Alpha-amylase variants
AssigneeNovozymes A/S
InventorsCarsten Andersen, Iben Damager, Chakshusmathi Ghadiyaram, Rajendra Kulothungan Sainathan, Padma Venkatachalam Iyer
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Abstract
The present invention relates to variants of a parent alpha-amylase having an improved wash performance when compared to the parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and method of producing the variants of the present invention.
Claims16
A variant of a parent alpha-amylase, wherein said variant comprises:
- (i) substitutions corresponding to H1*, G7A, N280S, and E391A, wherein the amino acid numbering corresponds to the positions of the amino acid sequence set forth in SEQ ID NO:
- 1,
- (ii) at least 85% but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, or 8;
- (iii) has alpha-amylase activity, and
- (iv) has improved wash performance compared to the parent amino acid sequence set forth in SEQ ID NO:
- 2.
A method for obtaining an alpha-amylase variant, comprising introducing into a parent alpha-amylase substitutions corresponding to H1*, G7A, N280S, and E391A, wherein the amino acid numbering corresponds to the positions of the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said parent alpha-amylase has at least 85% but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, or 8, and wherein said variant has alpha-amylase activity;
- and recovering said variant.
Dependencies are inferred from the claim text.