US 12,753,394 B2 · Composition for enhancing reprogramming efficiency from somatic cell to induced pluripotent stem cell, comprising mTOR activator, and method for enhancing reprogramming efficiency by using same

Abstract

The present invention relates to a composition for enhancing reprogramming efficiency from somatic cells to induced pluripotent stem cells, comprising an mTOR activator, and a method for enhancing reprogramming efficiency by using same. In the method, reprogramming factors including OCT4, SOX2, c-Myc, and KLF4 are transduced into somatic cells, followed by treatment with an mTOR activator, thereby remarkably increasing reprogramming efficiency into induced pluripotent stem cells. Therefore, the composition and method can be used for effectively inducing the reprograming of somatic cells into induced pluripotent stem cells.

Claims4

Dependent claims

A method for enhancing reprogramming efficiency from somatic cells to induced pluripotent stem cells, the method comprising:

a somatic transformation step of transducing one or more nucleic acid molecules encoding for all of octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), cellular myelocytomatosis oncogene (c-Myc), and Kruppel-like factor 4 (KLF4) into somatic cells;
and
an incubating step of incubating the transformed somatic cells in a culture medium comprising 4,6-di-4-morpholinyl-N-(4-nitrophenyl)-1,3,5-triazin-2-amine (MHY1485) and a placenta-derived cell conditioned medium (PCCM).

Dependencies are inferred from the claim text.

Composition for enhancing reprogramming efficiency from somatic cell to induced pluripotent stem cell, comprising mTOR activator, and method for enhancing reprogramming efficiency by using same

Life of this patent

Composition for enhancing reprogramming efficiency from somatic cell to induced pluripotent stem cell, comprising mTOR activator, and method for enhancing reprogramming efficiency by using same

Life of this patent

Abstract

Claims4