High-purity mesenchymal stem cells
AssigneePurec Co., Ltd.
InventorsYumi Matsuzaki, Takashi Suyama
Life of this patent
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Abstract
A cell population of rapidly proliferating mesenchymal stem cell clones that are positive for LNGFR (CD271) or co-positive for LNGFR (CD271) and Thy-1 (CD90), wherein at least one of the following characteristics (a) and (b) is satisfied. (a) The variation coefficient of forward scattered light in flow cytometry is 35% or less. (b) The average cell size is 20 μm or less.
Claims8
A cell population of rapidly proliferating mesenchymal stem cell clones that are positive for LNGFR (CD271) or that are double-positive for LNGFR (CD271) and Thy-1 (CD90), wherein the average cell diameter is 20 μm or less and the coefficient of variation of the forward scatter of the cell population in flow cytometry is 35% or less.
A culture of a cell population of human mesenchymal stem cells, wherein the cell population is obtained by a method comprising:
- (a) obtaining a cell population of mesenchymal stem cell clones that are positive for LNGFR (CD271) or that are double-positive for LNGFR (CD271) and Thy-1 (CD90);
- (b) single-cell culturing the mesenchymal stem cell clones;
- and
- (c) collecting rapidly expanding clones;
- wherein the average cell diameter is 20 μm or less and the coefficient of variation of the forward scatter in flow cytometry of the culture is 35% or less.
Dependencies are inferred from the claim text.