Methods of culturing quiescent hematopoietic stem cells and treatment methods
AssigneeIcahn School of Medicine at Mount Sinai
InventorsSaghi Ghaffari, Raymond Liang
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Abstract
The present disclosure relates to a method of culturing quiescent hematopoietic stem cells. This method involves providing a culture medium and introducing, into the culture medium, quiescent hematopoietic stem cells to culture the stem cells and maintain quiescence of the stem cells. The culture medium comprises a vacuolar-H+ adenosine triphosphate ATPase (“v-ATPase”) inhibitor. Also disclosed are methods of treating a subject for a hematological disorder, methods of culturing leukemic stem cells, and methods of enhancing the hematopoietic reconstitution ability of a population of human hematopoietic stem cells.
Claims16
A method of culturing quiescent hematopoietic stem cells, said method comprising:
- providing a serum-free culture medium comprising a vacuolar-H+ adenosine triphosphate ATPase (v-ATPase) inhibitor;
- introducing into the serum-free culture medium quiescent hematopoietic stem cells, wherein the stem cells are mitochondrial membrane potential (MMP)-low, lineage negative (Lin−), stem cell antigen 1 positive (Sca+), cKit30 , Signaling Lymphocyte Activation Molecule 1 positive (CD150+), and Signaling Lymphocyte Activation Molecule 2 negative (CD48−) cells;
- and
- culturing the quiescent hematopoietic stem cells in the serum-free culture medium to produce cultured quiescent hematopoietic stem cells, wherein said culturing is effective to maintain and promote the quiescence of the cultured quiescent hematopoietic stem cells.
Dependencies are inferred from the claim text.