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US 12,753,384 B2Granted
Methods and compositions for promoting survival and proliferation of endothelial cells and stimulating angiogenesis
AssigneesCornell Research Foundation, Inc., Sloan-Kettering Institute for Cancer Research
InventorsShahin Rafii, Fan Zhang, Marco Seandel
Life of this patent
Estimating the term…
Abstract
The present invention relates to adenovirus E4ORF1 gene and to endothelial cells engineered to express the E4ORF1 gene. The present invention also relates to uses of the E4ORF1 gene, and cells expressing the E4ORF1 gene, and to compositions comprising the E4ORF1 gene, or comprising cells expressing the E4ORF1 gene.
Claims14
A method for maintaining or expanding endothelial cells in culture, comprising:
- (a) introducing into primary endothelial cells a nucleic acid molecule comprising the adenovirus E4ORF1 gene, unaccompanied by the adenovirus E4ORF2 to E4ORF6 genes, under the control of a promoter to produce E4ORF1-expressing endothelial cells, and
- (b) culturing the E4ORF1-expressing endothelial cells, wherein the culturing comprises performing one or more serial passages of the cells.
Dependencies are inferred from the claim text.