Cell separation apparatus for bioreactor
AssigneeAlit Biotech (Shanghai) Co., Ltd.
InventorsYu Liu, Rui Chen
Life of this patent
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Abstract
The present disclosure provides a cell separation apparatus for a bioreactor, which comprises: a cell separation component, which is arranged in a tank and immersed below the liquid level of the mixture and includes a filter membrane and a liquid guiding groove; and a tangential flow driving component, which includes one or more blades. The cell separation apparatus implements a low shear force and the filter membrane is not blocked easily. The present disclosure also provides a string system including a central shaft and a multi-layer paddle component. The multi-layer paddle component includes an upper paddle component, an intermediate paddle component and a bottom paddle component arranged around the central shaft.
Claims20
A cell separation apparatus for a bioreactor comprising a tank for containing a mixture of cells and a liquid, wherein the cell separation apparatus comprises:
- a cell separation component and a tangential flow driving component, wherein
- the cell separation component is arranged in a tank and immersed below a liquid level of the mixture and include a filter membrane and a liquid guiding groove, wherein
- the filter membrane is configured to filter the mixture and guide a separated liquid into the liquid guiding groove,
- the liquid guiding groove comprises a radial outer wall and a radial inner wall, and an internal cavity of the liquid guiding groove is only in fluid communication with the tank through one or more openings on the radial inner wall and through the filter membrane, or only in fluid communication with the tank through one or more openings on the radial outer wall and through the filter membrane;
- and
- the tangential flow driving component comprises one or more blades positioned radially inside the cell separation component and capable of rotating about a rotation shaft to drive the mixture to flow tangentially along the filter membrane.
A bioreactor, comprising a cell separation apparatus and a tank used for containing a mixture of cells and a liquid, wherein the cell separation apparatus comprises:
- a cell separation component and a tangential flow driving component, wherein
- the cell separation component is arranged in a tank and immersed below a liquid level of the mixture and include a filter membrane and a liquid guiding groove, wherein
- the filter membrane is configured to filter the mixture and guide a separated liquid into the liquid guiding groove,
- the liquid guiding groove comprises a radial outer wall and a radial inner wall, and an internal cavity of the liquid guiding groove is only in fluid communication with the tank through one or more openings on the radial inner wall and through the filter membrane, or only in fluid communication with the tank through one or more openings on the radial outer wall and through the filter membrane;
- and
- the tangential flow driving component comprises one or more blades positioned radially inside the cell separation component and capable of rotating about a rotation shaft to drive the mixture to flow tangentially along the filter membrane.
Dependencies are inferred from the claim text.