High-yield peroxide quench-controlled polysaccharide depolymerization and compositions thereof
AssigneeOne Bio Inc
InventorsMatthew Joseph Amicucci, Cory Glen Vierra, Riley Anne Drexler, Kevin Patrick Worrell, Yiyun Liu
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Abstract
Provided are methods for cleaving polysaccharides, comprising reacting polysaccharides with a Fenton's reagent, and cleaving the treated polysaccharides with a nitrogen-based cleavage reagent, which preferably is also a peroxide-quenching agent. Synthetic oligosaccharide compositions produced by such polysaccharide cleaving methods are further disclosed. Such oligosaccharide compositions are shown to provide utility in various aspects including modulating microbial growth and/or microbial or host metabolism.
Claims20
A synthetic oligosaccharide composition comprising:
- one or more oligosaccharides,
- wherein each of the oligosaccharides independently comprises between 3 and 6 galactose monomers, wherein at least a portion of the oligosaccharides further comprises one or more arabinose monomers,
- wherein the synthetic oligosaccharide composition has a glycosidic linkage composition including terminal galactose, β-1,3 galactose, β-1,3,6 galactose, β-1,6 galactose, or terminal arabinose, and
- wherein the synthetic oligosaccharide composition is generated by reacting a polysaccharide having a β-1,3 galactose backbone in a reaction mixture with a Fenton's reagent comprising a peroxide agent and metal ions to provide a treated polysaccharide, and cleaving the treated polysaccharide with a nitrogen-based cleavage reagent to generate the oligosaccharides.
A synthetic oligosaccharide composition comprising oligosaccharides having a β-1,3 galactose backbone,
- wherein each of the oligosaccharides independently comprises 3 to 6 galactose monomers, wherein at least a portion of the oligosaccharides further comprises one or more arabinose monomers, and
- wherein the synthetic oligosaccharide composition has a glycosidic linkage composition consisting of terminal galactose, β-1,3 galactose, β-1,3,6 galactose, β-1,6 galactose, or terminal arabinose.
A synthetic oligosaccharide composition comprising oligosaccharides having a β-1,3 galactose backbone,
- wherein each of the oligosaccharides independently comprises 3 to 6 galactose monomers, and wherein at least a portion of the oligosaccharides further comprises one or more arabinose monomers,
- wherein the synthetic oligosaccharide composition has a glycosidic linkage composition including terminal galactose, β-1,3 galactose, β-1,3,6 galactose, β-1,6 galactose, or terminal arabinose, and
- wherein the oligosaccharides of the synthetic oligosaccharide composition exhibit the mass and retention time values in Table 11.
Dependencies are inferred from the claim text.