US 12,753,241 B2 · Anti-ABCG2 antibodies and uses thereof

Abstract

Provided are antibodies that target the cellular efflux pump ABCG2. Also provided are pharmaceutical compositions, nucleic acids, recombinant expression vectors, cells, and kits that include or encode such antibodies. Methods of using the antibodies for detecting presence or absence of ABCG2 expression in cells, e.g., tumor cells, level of ABCG2 expression, and/or inhibiting ABCG2 function are also disclosed. In addition, multi-specific antibodies that bind to ABCG2 and a polypeptide expressed on surface of a cancer cell are provided. The polypeptide may be the efflux pump MDR1 or a cancer-associated antigen. Also provided are methods for treating a subject for a cancer that include administering to the subject an anti-ABCG2 antibody as disclosed herein or a multi-specific antibody that targets both ABCG2 and a cancer-associated antigen or MDR1.

Claims17

Dependent claims

A bispecific antibody molecule that binds ATP Binding Cassette Subfamily G Member 2 (ABCG2) and a tumor associated antigen (TAA), wherein the TAA is EGFR or PD-L1,

the bispecific antibody molecule comprising two identical variable light (VL) chains, a first variable heavy (VH) chain, and a second VH chain, wherein
the VL chains each comprise an antigen-binding site for ABCG2 comprising LCDRs 1-3:
KAGQNINNYLA (SEQ ID NO:273);
NANSLQT (SEQ ID NO:309);
and QQYNSWTT (SEQ ID NO:337);
the first VH chain comprises an antigen-binding site for ABCG2 comprising HCDRs 1-3:
SGYDWS (SEQ ID NO:87);
YISYSGWTNYNPSLRS (SEQ ID NO:119);
and VRGYNPFAY (SEQ ID NO:160);
and
the second VH chain comprises an antigen-binding site for the EGFR comprising HCDRs 1-3:
GGSISSGDY (SEQ ID NO:421);
YYSGS (SEQ ID NO:422);
VSIFGVGTFDY (SEQ ID NO:423);
or
the second VH chain comprises an antigen-binding site for the PD-L1 comprising HCDRs 1-3:
GFTFSDS (SEQ ID NO:424);
SPYGGS (SEQ ID NO:425);
RHWPGGFDY (SEQ ID NO:426).

16 more elements

Dependencies are inferred from the claim text.

Anti-ABCG2 antibodies and uses thereof

Life of this patent

Anti-ABCG2 antibodies and uses thereof

Life of this patent

Abstract

Claims17