Compositions and methods for genetically-encoded high voltage-activated calcium channel blockers using engineered ubiquitin ligases
AssigneeThe Trustees of Columbia University in the City of New York
InventorsTravis Morgenstern, Henry M. Colecraft
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Abstract
The present disclosure provides, inter alia, nanobodies targeting a CaVβ auxiliary subunit and compositions thereof. Methods for blocking a High-Voltage Activated Calcium Channel (HVACC) using such compositions, methods for selectively targeting a population of cells in a subject, and methods for treating or ameliorating the effects of a disease in a subject are also provided.
Claims14
A composition comprising a nucleic acid molecule encoding a polypeptide comprising (i) an E3 ubiquitin ligase operably connected to (ii) a nanobody capable of binding to a Cavβ auxiliary subunit, wherein the amino acid sequence of the encoded nanobody comprises three complementarity determining regions (CDRs):
- CDR1, CDR2, and CDR3, and wherein the amino acid sequence of CDR1 comprises the amino acid sequence of SEQ ID NO:
- 83, the amino acid sequence of CDR2 comprises the amino acid sequence SEQ ID NO:
- 84, and the amino acid sequence of CDR3 comprises the amino acid sequence of SEQ ID NO:
- 85.
Dependencies are inferred from the claim text.