Polypeptide exhibiting fluorescent properties and use for same
AssigneesRiken, Tohoku University
InventorsAtsushi Miyawaki, Ryoko Ando, Masahiko Hirano, Noriyo Takeda
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Abstract
A novel fluorescent protein and uses thereof are provided. A polypeptide of the present invention has a fluorescence property, the polypeptide being indicated in any one of (1) to (3) below: (1) a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1; (2) a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1, the amino acid sequence having replaced, deleted, inserted, and/or added therein not less than 1 and not more than 32 amino acids; and (3) a polypeptide having not less than 85% sequence identity with respect to an amino acid sequence set forth in SEQ ID NO: 1.
Claims13
A polypeptide having a fluorescence property, the polypeptide comprising any one of (1) to (3) below:
- (1) a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1;
- (2) a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1, the amino acid sequence having replaced, deleted, inserted, and/or added therein not less than 1 and not more than 32 amino acids, wherein amino acid 168 is an alanine and which is not permitted to be substituted;
- and
- (3) a polypeptide having not less than 85% sequence identity with respect to the amino acid sequence set forth in SEQ ID NO:
- 1, wherein amino acid 168 is an alanine and which is not permitted to be substituted.
A polynucleotide indicated in any one of (1) to (3) below:
- (1) a polynucleotide which encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1;
- (2) a polynucleotide which encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1, the amino acid sequence having replaced, deleted, inserted, and/or added therein not less than 1 and not more than 32 amino acids, the polypeptide having a fluorescence property, wherein amino acid 168 is an alanine and which is not permitted to be substituted;
- and
- (3) a polynucleotide which encodes a polypeptide having not less than 85% sequence identity with respect to the amino acid sequence set forth in SEQ ID NO:
- 1 and having a fluorescence property, wherein amino acid 168 is an alanine and which is not permitted to be substituted.
A fusion polypeptide comprising:
- a polypeptide having a fluorescence property;
- and another polypeptide,
- the fusion polypeptide including not less than two polypeptides linked together each of which is a polypeptide indicated in any one of (1) to (3) below:
- (1) a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1;
- (2) a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 1, the amino acid sequence having replaced, deleted, inserted, and/or added therein not less than 1 and not more than 32 amino acids, wherein amino acid 168 is an alanine and which is not permitted to be substituted;
- and
- (3) a polypeptide having not less than 85% sequence identity with respect to the amino acid sequence set forth in SEQ ID NO:
- 1, wherein amino acid 168 is an alanine and which is not permitted to be substituted.
Dependencies are inferred from the claim text.