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US 12,753,197 B2Granted
Colour tunable luminescent bidentate platinum (II) complexes for probing mismatch DNA
AssigneeThe University of Hong Kong
InventorsChi Ming Che, Ming Zhang, Chun Nam Lok
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Abstract
Pertains to the design and applications of platinum (II) compounds supported by a bidentate and N-heterocyclic carbene ligands. The Pt (II) complexes exhibit strong emission intensity differences when contacted with matched and mismatched DNA. In addition, the Pt (II) complexes show a color tunable effect when exposed to mismatched compared to matched DNA, which color effect can be easily detected.
Claims16
A method for quantifying DNA mismatches in a sample, the method comprising:
- providing a sample suspected to comprise DNA mismatches and digesting the DNA in the sample with restriction enzymes to obtain DNA molecules comprising between 20 and 200 nucleotides;
- providing a sample of matched DNA molecules comprising between 20 and 200 nucleotides;
- contacting the samples with a concentration of a Pt (II) complex comprising a bidentate group, a halogen group, and a N-heterocyclic group, wherein the N-heterocyclic group further comprises a hetero substituted imidazolium group such that the ratio of DNA molecules to Pt (II) complex is at least 1;
- measuring emission spectra of the samples after contacting them with the Pt (II) complex;
- quantifying the orange-red and blue-green emission intensities in the emission spectra;
- and
- quantifying the number of DNA mismatches in the sample suspected to comprise DNA mismatches based on the orange-red and blue-green emission intensity of the sample;
- wherein the blue-green emission intensity is equal to or less than the orange-red intensity in the sample of matched DNA molecules;
- wherein a blue-green emission intensity that is greater than the orange-red intensity indicates the presence of DNA mismatches in the sample suspected to comprise DNA mismatches;
- and
- wherein the blue-green emission intensity is proportional to the number of DNA mismatches in the DNA molecules of the sample suspected to comprise DNA mismatches.
8 more elements
A method for quantifying DNA mismatches in a sample, the method comprising:
- providing a sample suspected to comprise DNA mismatches and digesting the DNA in the sample with restriction enzymes to obtain DNA molecules comprising between 20 and 200 nucleotides;
- providing a sample comprising matched DNA molecules comprising between 20 and 200 nucleotides;
- contacting the samples with a concentration of a Pt (II) complex comprising a bidentate group, a halogen group, and a N-heterocyclic group, wherein the N-heterocyclic group further comprises a hetero substituted imidazolium group such that the ratio of DNA molecules to Pt (II) complex is at least 1;
- contacting the samples with UV light;
- and
- determining the color of the sample;
- wherein the color of the sample is orange in the sample comprising matched DNA molecules;
- and a blue-green color in the sample suspected to comprise DNA mismatches indicates the presence of DNA mismatches;
- wherein the intensity of the blue-green color in the sample suspected to comprise DNA mismatches is proportional to the number of DNA mismatches in the DNA molecules of said sample.
6 more elements
Dependencies are inferred from the claim text.