Sequencing systems including a base unit and removable cartridge
AssigneeEGI Tech (Qing Dao) Co., Limited
InventorsSz-Chin Lin, Cheng Frank Zhong, Yiwen Ouyang, Sixing Li, Daqing Liu
Life of this patent
Estimating the term…
Abstract
Embodiments include systems for sequencing a biological sample. The system may include a reusable subsystem and a removable subsystem. The reusable subsystem may actuate and operate the removable subsystem to automate the sequencing. A base unit of the reusable subsystem may form a fluidic connection between an integrated reagent cartridge and an integrated sensor cartridge of the removable subsystem. The integrated reagent cartridge may be configured to hold reagents and the integrated sensor cartridge may be configured with a biosensor for sequencing the biological sample.
Claims23
A sequencing system, the system comprising:
- (a) a removable integrated reagent cartridge (IRC), the IRC including a cartridge external housing enclosing one or more reservoirs for holding one or more sequencing reagents, the IRC further including one or more connectors in fluid communication with the one or more reservoirs, the one or more connectors comprising a plurality of reagent seals at the external housing and at least one pump line connector at the external housing;
- (b) a removable integrated sensor cartridge (ISC), the ISC including:
- (i) a plurality of reagent receiving ports, the reagent receiving ports located to fluidically connect to the reagent seals of the IRC when the IRC is brought into engagement with the ISC;
- (ii) at least one biological sample input;
- (iii) a reaction chamber including at least one sensor comprising a functionalized surface and an array of detectors configured to detect biological analytes on or near the functionalized surface;
- (iv) a fluidic network configured to selectively fluidically connect the reagent receiving ports and biological sample input to the reaction chamber;
- and
- (c) a base unit configured to removably receive the IRC and the ISC, wherein the IRC and ISC are configured to be compressed together when loaded into the base unit, the base unit configured to control sequencing reactions in the reaction chamber and to receive sequencing data from the sensor when the IRC and ISC are loaded into the base unit.
A sequencing system, the system comprising:
- (a) a removable integrated reagent cartridge (IRC) configured to hold one or more sequencing reagents;
- (b) a removable integrated sensor cartridge (ISC) comprising a reaction chamber and at least one valve, the reaction chamber including at least one sensor electrically connected to a plurality of electrical contacts;
- and
- (c) a base unit, the system configured for removable installation of the IRC and ISC in the base unit, the base unit comprising a valve actuator and an electric connector assembly,
- wherein the base unit is configured such that upon installation of the IRC and ISC in the base unit, the base unit compresses the IRC and ISC together such that the IRC is fluidically connected to the ISC, the valve actuator is operably engaged to the at least one valve of the ISC, and the electric connector assembly is electrically coupled to the plurality of electrical contacts of the ISC.
Dependencies are inferred from the claim text.