Microstructure and molecular detection method

A hemispherical shell-shaped hollow-multilayered microstructure for use in detection of a target molecule, comprising:

• a first thin film layer in the form of a substantially micro-hemispherical shell composed of a first material comprising a magnetic material, and
• a second thin film layer disposed on the inner surface of the micro-hemispherical shell and composed of a second material, wherein said second material is capable of removably fixing a fluorochrome-labeled probe and causing fluorescence resonance energy transfer between the fluorochrome and the second material, wherein the second material is nanocarbon in which an SP2 bonded region and an SP3 bonded region are mixed,
• wherein a hollow space defined by the second thin film layer has a size that is capable of capturing at least one cell of the target molecule or a portion thereof in said hollow space, and
• wherein the fluorochrome-labeled probe is a molecule capable of specific binding to the target molecule, and said binding to the target molecule can alter the structure of the fluorochrome-labeled probe, thereby causing a change from quenching to emission of the fluorochrome,
• wherein the hemispherical shell-shaped hollow multilayer microstructure comprises said fluorochrome-labeled probe removably fixed to a surface of said second thin film layer,
• wherein the fluorochrome-labeled probe is a nucleic acid molecule modified with the fluorochrome at one end and a pyrene molecule at the other end, and
• wherein the fluorochrome is bound to the fluorochrome-labeled probe via a spacer molecule.