Back to results
US 12,752,220 B2Granted
Column comprising particles
AssigneeAgilent Technologies, Inc.
InventorsTa-Chen Wei, Andrei Bordunov, Charu Kumar, Ace Gita Galermo, John Davies, Andrew Coffey
Life of this patent
Estimating the term…
Abstract
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
Claims20
A chromatography column, comprising:
- particles having an average particle size ranging from about 1 μm to about 5 μm;
- an average pore size ranging from about 450 Å to about 3000 Å;
- an average pore volume ranging from about 1 cm3/g to about 5 cm3/g;
- and
- a particle size distribution of less than about 20% of the average particle size.
2 more elements
A method of using a size exclusion chromatography column, comprising:
- injecting a viral analyte into a column comprising particles having an average particle size ranging from about 1 μm to about 5 μm;
- an average pore size ranging from about 450 Å to about 3000 Å;
- and an average pore volume ranging from about 1 cm3/g to about 5 cm3/g;
- and
- operating the column at an adjustable flow rate of between about 0.1 ml/min and about 0.7 ml/min to achieve chromatographic separation of the viral analyte at a resolution of at least 1.2.
2 more elements
Dependencies are inferred from the claim text.