Lipid nanoparticles comprising sterols and mRNA
AssigneeseTheRNA Immunitherapies NV, Vrije Universiteit Brussel
InventorsSanne Bevers, Stefaan De Koker, Raymond Michel Schiffelers, Sander Alexander Antonius Kooijmans
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of lipid nanoparticles (LNP); more specifically comprising an ionizable lipid, a phospholipid, a sterol, a PEG lipid and one or more nucleic acids. The LNP's of the present invention are characterized in comprising less than about 1 mol % of a C14-PEG2000 lipid; as well as particular percentages of the other lipids. The present invention provides use of the LNP's for immunogenic delivery of nucleic acid molecules, specifically mRNA; thereby making them highly suitable for use in vaccines, such as for the treatment of cancer or infectious diseases. Finally, methods are provided for preparing such LNP's.
Claims18
A lipid nanoparticle comprising:
- an ionizable lipid;
- a phospholipid;
- a sterol;
- a PEG lipid;
- and
- one or more mRNA molecules,
- wherein:
- the PEG lipid is a C14-PEG lipid;
- the lipid nanoparticle comprises from about 0.5 mol % to about 0.9 mol % of the PEG lipid;
- the molar percentage of the ionizable lipid is from about 55 mol % to about 60 mol %;
- the molar percentage of the phospholipid is from about 5 mol % to about 10 mol %;
- and
- the molar percentage of the sterol is from about 25 mol % to about 39.5 mol %.
A lipid nanoparticle, comprising:
- about 65 mol % of the ionizable lipid;
- about 9.5 mol % DOPE;
- about 25 mol % cholesterol;
- about 0.5 mol % DMG-PEG2000;
- and
- one or more mRNA molecules.
Dependencies are inferred from the claim text.