US 12,751,460 B2 · Composition targeting V-ATPase for controlling Frankliniella occidentalis and method of controlling Frankliniella occidentalis using same

Abstract

Proposed are a composition for controlling Frankliniella occidentalis by oral feeding, in which a dsRNA specific to V-ATPase in Frankliniella occidentalis is formulated into a liposomal form and a method of controlling Frankliniella occidentalis using the same. The composition contains a dsRNA specific to V-ATPase in Frankliniella occidentalis and a carrier to formulate the dsRNA in a liposomal form, in which Frankliniella occidentalis is controlled by oral feeding. When orally feeding the composition to larvae and adults of Frankliniella occidentalis, the dsRNA is prevented from being degraded by dsRNase in the intestinal lumen of insects. Therefore, with the maintained RNAi effect, the corresponding gene expression can be effectively inhibited, thereby exhibiting excellent insecticidal effects. In addition, excellent control effects are obtainable by oral feeding, a simple method involving a process of spraying the composition onto host plants attacked by Frankliniella occidentalis.

Claims4

Dependent claims

A composition for controlling Frankliniella occidentalis comprising:

(a) a double-stranded RNA (dsRNA) specific to one or more subunits selected from the group consisting of V-ATPase-A, V-ATPase-C, V-ATPase-D, V-ATPase-E, V-ATPase-F, V-ATPase-G, and V-ATPase-H, excluding V-ATPase-B,
wherein each subunit-specific dsRNA corresponds to a double-stranded nucleotide sequence defined by a primer pair as follows:
SEQ ID NOs:
1 and 2 for subunit A;
SEQ ID NOs:
5 and 6 for subunit C;
SEQ ID NOS:
7 and 8 for subunit D;
SEQ ID NOs:
9 and 10 for subunit E;
SEQ ID NOs:
11 and 12 for subunit F;
SEQ ID NOS:
13 and 14 for subunit G;
and
SEQ ID NOs:
15 and 16 for subunit H;
and
(b) a liposomal carrier comprising a cationic lipid N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride and a neutral lipid dioleoylphosphatidylethanolamine,
wherein the composition is formulated for oral administration by spraying onto host plants consumed by the insect.

17 more elements

Dependencies are inferred from the claim text.

Composition targeting V-ATPase for controlling Frankliniella occidentalis and method of controlling Frankliniella occidentalis using same

Life of this patent

Composition targeting V-ATPase for controlling Frankliniella occidentalis and method of controlling Frankliniella occidentalis using same

Life of this patent

Abstract

Claims4