Methods for the preparation of non-live Chromobacterium biopesticide
AssigneeThe Johns Hopkins University
InventorsGeorge Dimopoulos, Eric Caragata, Raul Saraiva
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Abstract
The present invention relates to the field of pesticides. More specifically, the present invention provides compositions and methods for the preparation of non-live Chromobacterium pesticides. In a specific embodiment, a method for producing a non-live Chromobacterium biopesticide comprises the steps of (a) growing the Chromobacterium in a bioreactor with culture medium; (b) oxygenating the Chromobacterium during step (a); (c) adding an antibiotic to the Chromobacterium culture; (d) incubating the Chromobacterium culture under hypoxic conditions; (e) pouring the Chromobacterium culture on to a substrate; (f) air drying the Chromobacterium; and (g) crushing the dried Chromobacterium into a powder.
Claims13
A method for producing a non-live Chromobacterium biopesticide comprising the steps of:
- (a) growing the Chromobacterium in a bioreactor with culture medium;
- (b) oxygenating the Chromobacterium during step (a);
- (c) adding an antibiotic to the Chromobacterium culture;
- (d) incubating the Chromobacterium culture under hypoxic conditions;
- (e) pouring the Chromobacterium culture on to a substrate;
- (f) air drying the Chromobacterium;
- and
- (g) crushing the dried Chromobacterium into a powder.
A method for producing a non-live Chromobacterium biopesticide comprising the steps of:
- (a) growing the Chromobacterium in a bioreactor with culture medium;
- (b) oxygenating the Chromobacterium during step (a);
- (c) adding an antibiotic to the Chromobacterium culture;
- (d) incubating the Chromobacterium culture under hypoxic conditions;
- (e) performing an organic extraction of the Chromobacterium;
- and
- (f) collecting the organic phase comprising the Chromobacterium.
Dependencies are inferred from the claim text.