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US 12,748,294 B2Granted
Substrates for performing quantification processes and related systems and methods
AssigneeIllumina, Inc.
InventorBryan Crane
Life of this patent
Estimating the term…
Abstract
Substrates for performing quantification processes and related systems and methods are disclosed. In an implementation, an apparatus includes a substrate and an imaging system. The substrate includes a pair of plates and a plurality of spacers positioned between the plates to define a gap between the pair of plates. A portion of a sample is to be received within the gap of the substrate and the imaging system is to obtain image data of the portion of the sample. The image data is to be used to determine a concentration of the portion of the sample.
Claims29
An apparatus, comprising:
- a contact dispenser;
- a stage coupled to and used to move the contact dispenser;
- a substrate comprising a pair of plates and a plurality of spacers positioned between the plates to define a gap between the pair of plates, wherein the substrate comprises an inlet and an outlet in fluid communication with the gap, the outlet opening downwardly to waste;
- and
- an imaging system,
- wherein, to perform a quantification process, the stage aligns the contact dispenser with the inlet of the substrate and the contact dispenser dispenses a portion of a sample into the inlet of the substrate, and wherein the imaging system is to obtain image data of the portion of the sample, the image data to be used to determine a concentration of the portion of the sample.
3 more elements
A method, comprising:
- performing a quantification process, comprising:
- aligning a contact dispenser using a stage with a first plate of a substrate;
- dispensing, using the contact dispenser, a sample onto the first plate of the substrate;
- positioning a second plate of the substrate on the first plate and defining a gap between the first plate and the second plate using a plurality of spacers wherein the spacers comprise ball bearings having opposing flat surfaces;
- obtaining image data of the sample;
- and
- determining a concentration of the sample using the image data.
4 more elements
A method, comprising:
- performing a quantification process, comprising:
- aligning a contact dispenser using a stage with an inlet of a channel of a substrate;
- dispensing a sample in the inlet of the channel of the substrate using the contact dispenser;
- obtaining image data of the sample;
- determining a concentration of the sample using the image data;
- and
- flowing the sample downwardly out of an outlet of the channel to waste.
4 more elements
An apparatus, comprising:
- a contact dispenser;
- a stage coupled to and used to move the contact dispenser;
- a substrate, comprising:
- a channel including an inlet and an outlet;
- and
- a valve disposed between the inlet and the outlet, wherein the channel comprises a first portion between the valve and the inlet and a second portion between the valve and the outlet;
- and
- an imaging system,
- wherein, to perform a quantification process, the stage aligns the contact dispenser with the inlet of the substrate and the contact dispenser dispenses a portion of a sample into the inlet of the substrate, and wherein the imaging system is to obtain image data of the portion of the sample, the image data to be used to determine a concentration of the portion of the sample.
6 more elements
An apparatus, comprising:
- a substrate, comprising:
- a channel including an inlet and an outlet;
- and
- a valve disposed between the inlet and the outlet, wherein the channel comprises a first portion between the valve and the inlet and a second portion between the valve and the outlet;
- a pump to move the sample from the inlet to the outlet;
- and
- an imaging system,
- wherein a portion of a sample is to be received within the first portion of the channel of the substrate and the imaging system is to obtain image data of the portion of the sample, the image data to be used to determine a concentration of the portion of the sample, wherein the pump comprises a plate comprising an axis of rotation and an actuator to rotate the plate, the substrate coupled to the plate and extending radially from the axis of rotation.
5 more elements
Dependencies are inferred from the claim text.