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US 12,748,120 B2Granted
HIV viral load testing
AssigneeDiagnostics for the Real World, Ltd.
InventorJean-Pierre Allain
Life of this patent
Estimating the term…
Abstract
Methods of testing HIV viral load are described. The methods comprise detecting HIV viral RNA in a sample of leukocyte-depleted blood. Such methods can be carried out on low-volume samples obtained without the need for venipuncture or a centrifuge. The methods are particularly suited for HIV viral load testing in resource-limited settings. Methods for monitoring HIV infection are also described, as well as kits for carrying out the methods.
Claims11
A kit for HIV viral load testing, which comprises:
- a leukoreduction filter which depletes at least 99.9% leukocytes from a whole blood sample or a diluted whole blood sample, and leaves at least 50% of the red blood cells present in the whole blood sample or the diluted whole blood sample remaining in the whole blood sample or diluted whole blood sample after filtration, wherein the leukoreduction filter is a depth filter or a screen filter having an average dead volume of 39.2 μl to 77.2 μl;
- a blood collector with up to 500 μl capacity for collecting a low volume whole blood sample;
- a means for applying a pressure differential across the leukoreduction filter;
- and
- reagents required for reverse transcription of HIV viral RNA, and subsequent nucleic acid amplification of a product of the reverse transcription, wherein the reagents required for amplification comprise primers for specific amplification of HIV nucleic acid.
2 more elements
A kit for HIV viral load testing, which comprises:
- a leukoreduction filter which depletes at least 99.9% of leukocytes from a whole blood sample or a diluted whole blood sample and leaves at least 50% of the red blood cells present in the whole blood sample or the diluted whole blood sample remaining in the whole blood sample or diluted whole blood sample after filtration, wherein the leukoreduction filter is a depth filter or a screen filter having an average dead volume of 39.2 μl to 77.2 μl;
- a means for applying pressure across the leukoreduction filter;
- reagents required for reverse transcription of HIV viral RNA, and subsequent nucleic acid amplification of a product of the reverse transcription;
- and
- a lancet for obtaining a low volume sample of whole blood from a subject by finger prick or heel prick.
2 more elements
Dependencies are inferred from the claim text.