POCT diagnostic system for traumatic brain injuries and infectious diseases
AssigneeEzdiatech Inc.
InventorsYong Gyun Jung, Joo Ho Kim
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Abstract
The present invention relates to a POCT diagnostic system that can quickly identify traumatic brain injuries and infectious diseases and can accurately determine traumatic brain injuries and infectious diseases with high sensitivity. The present invention provides magnetic particles including a core including a magnetically responsive metal, a shell layer surrounding the core and having a uniform thickness, and capture probes introduced onto the shell layer to capture biological materials. Each of the capture probes includes an antibody against a biomarker for the diagnosis of a traumatic brain injury or infectious disease as an antigen.
Claims15
Magnetic particles comprising:
- a core comprising a magnetically responsive metal,
- a shell layer surrounding the core and having a uniform thickness of 4 to 8 μm, and
- capture probes immobilized on a surface of the shell layer to capture biological materials, wherein,
- the shell layer is treated with an acidic solution comprising a mixture of a strong acid and hydrogen peroxide in a volume ratio of 1:1 to 7:1,
- the shell layer has an average surface roughness (Ra) of 1 to 15 nm,
- the surface of the shell layer has hydrophilic functional groups at a density of 1 to 80 groups per nm2;
- and
- the capture probes are linked to the hydrophilic functional groups on the surface of the shell layer,
- wherein each of the capture probes comprises an antibody against a biomarker for the diagnosis of a traumatic brain injury or infectious disease as an antigen.
A method for preparing magnetic particles comprising:
- (i) preparing magnetic micro rods comprising a core comprising a magnetic material and a shell layer made of glass and having a uniform thickness of 4 to 8 μm;
- (ii) treating a surface of the magnetic micro rods with an acidic solution comprising a mixture of a strong acid and hydrogen peroxide in a volume ratio of 1:1 to 7:1 to form silanol groups on the surface of the shell layer;
- (iii) immersing the surface-treated magnetic microrods micro rods-in a silane-based solution to bind silane molecules to the silanol groups;
- (iv) converting terminal groups of the silane molecules to functional groups capable of binding to probes;
- and
- (v) attaching probes to the functional groups,
- wherein,
- the shell layer obtained by the acidic treatment has an average surface roughness (Ra) of 1 to 15 nm, and
- the surface of the shell layer has hydrophilic functional groups at a density of 1 to 80 groups per nm2.
Dependencies are inferred from the claim text.