Chromatography devices and methods

A method of chromatographic analyte separation, comprising:

• a) inserting a chromatography pipette tip into a container of polar fluid, wherein said chromatography pipette tip is a pipette tip containing a hydrophilic filter and a dry chromatography resin above said hydrophilic filter, wherein said hydrophilic filter is positioned at a distal end of said pipette tip so as to have <10 μl dead volume below said hydrophilic filter;
• b) wetting said hydrophilic filter by capillary action pulling said polar fluid up through said hydrophilic filter and into said dry chromatography resin to form a swelled resin;
• c) equilibrating said swelled resin in said polar fluid for a time sufficient to form a homogenous wet resin (gel);
• d) optionally adding a small volume of said polar fluid to a top end of said chromatography pipette tip;
• e) draining any excess polar fluid out of said distal end of said chromatography pipette tip;
• f) adding a sample solution in said polar fluid to the top end of said chromatography pipette tip, said sample solution containing two or more analytes;
• g allowing said sample solution to load into said gel by gravity flow or by applying positive pressure;
• h) optionally adding a wash solution (void volume) to the top end of said chromatography pipette tip, and allowing said wash solution to flow via gravity or positive pressure;
• i) adding an elution solution (collection fraction) to the top end of said chromatography pipette tip;
• and
• j) collecting via gravity flow or positive pressure one or more elution fractions having separated analytes into one or more containers.

A chromatography pipette tip, comprising:

• a pipette tip comprising a hollow tube with an open top end over a conical section and a bottom end that is smaller than said top end;
• said top end sized to fit over a separate pipette head;
• an exterior surface of said pipette tip above said conical section having an annular ridge or three or more vertical fins;
• a hydrophilic filter and a dry chromatography resin or media above said hydrophilic filter;
• said hydrophilic filter positioned at said bottom end of said pipette tip so as to have less than 10 μl dead volume inside said pipette tip and below said hydrophilic filter;
• and
• a barrier at said top end to retain said dry chromatography resin.