US 12,747,479 B2 · Compositions and methods for detection of malaria

Abstract

Methods for the rapid detection of the presence or absence of Malaria parasites (including Plasmodium) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Additionally, the assay can be a multiplex assay, to amplify and detect a plurality of Plasmodium targets simultaneously, which offers advantages over singleplex assays. Furthermore, oligonucleotide primers and oligonucleotide probes targeting Malaria parasites (including Plasmodium) and kits are provided that are designed for the detection of Plasmodium, including, but not limited to, the Plasmodium species of P. falciparum, P. vivax, P. ovale, P. knowlesi, and P. malariae. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Malaria parasites (including Plasmodium).

Claims20

Dependent claims

A method for detecting one or more Malaria parasite species in a sample, the method comprising:

(a) performing an amplification step comprising contacting the sample with one or more set of primers to produce an amplification product, if a target nucleic acid of the one or more Malaria parasite species is present in the sample;
(b) performing a hybridization step comprising contacting the one or more probes with the amplification product, if a target nucleic acid of the one or more Malaria parasite species is present in the sample;
and
(c) detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of the one or more Malaria parasite species in the sample, and wherein the absence of the amplification product is indicative of the absence of the one or more Malaria parasite species in the sample;
and
wherein the one or more set of primers and the one or more probes comprises:
(1) primers comprising the nucleic acid sequences of SEQ ID NOs:
34, 35, and 36;
and a probe comprising the nucleic acid sequence of SEQ ID NO:
4, or a complement thereof;
and/or
(2) primers comprising the nucleic acid sequence of SEQ ID NOs:
56 and 57;
and a probe comprising the nucleic acid sequence of SEQ ID NO:
58, or a complement thereof.

12 more elements

A method for detecting a first target nucleic acid and/or a second target nucleic acid of one or more Malaria parasite species in a sample, the method comprising:

(a) performing an amplification step comprising contacting the sample with one or more set of primers to produce an amplification product, if the first target nucleic acid and/or the second target nucleic acid of the one or more Malaria parasite species is present in the sample;
(b) performing a hybridization step comprising contacting the one or more probes with the amplification product, if the first target nucleic acid and/or the second target nucleic acid of the one or more Malaria parasite species is present in the sample;
and
(c) detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of the one or more Malaria parasite species in the sample, and wherein the absence of the amplification product is indicative of the absence of the one or more Malaria parasite species in the sample;
and
wherein the one or more set of primers and the one or more probes comprises:
(1) a set of primers and a probe for the first target nucleic acid of the one or more Malaria parasite species, wherein the set of primers comprises primers comprising the nucleic acid sequences of SEQ ID NOs:
34, 35, and 36;
and wherein the probe comprises the nucleic acid sequence of SEQ ID NO:4, or a complement thereof;
and
(2) a set of primers and a probe for the second target nucleic acid of the one or more Malaria parasite species, wherein the set of primers comprises primers comprising the nucleic acid sequences of SEQ ID NOs:
56 and 57;
and wherein the probe comprises the nucleic acid sequence of SEQ ID NO:58, or a complement thereof.

10 more elements

Dependencies are inferred from the claim text.

Compositions and methods for detection of malaria

Life of this patent

Compositions and methods for detection of malaria

Life of this patent

Abstract

Claims20