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US 12,747,474 B2Granted
Densely-packed analyte layers and detection methods
AssigneeIllumina Cambridge Limited
InventorsNorman Burns, Dennis Ballinger
Life of this patent
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Abstract
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These methods and systems may have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.
Claims19
A method for determining a relative position of analytes deposited on a surface of a densely packed substrate, comprising:
- (a) providing a substrate comprising a surface, wherein said surface is patterned or unpatterned and comprises a plurality of analytes deposited on said surface at discrete locations;
- (b) performing a plurality of cycles of probe binding and signal detection on said surface, wherein a cycle of said plurality of cycles comprises:
- (i) contacting said plurality of analytes with a plurality of probes from a probe set, wherein a probe of said plurality of probes comprises a detectable label, wherein said probe binds to an analyte of said plurality of analytes;
- (ii) introducing said plurality of analytes to a solution comprising erythorbic acid;
- and
- (iii) imaging said surface with an optical unit to detect one or more optical signals from said probe bound to said analyte.
3 more elements
A method of controlling an average minimum center-to-center distance between one or more analytes, comprising:
- exposing a plurality of analytes deposited on a surface to a gas-liquid interface to form a monolayer of analytes deposited across said surface, said plurality of analytes treated with a repellant or attractive substance, and said plurality of analytes deposited on said surface at an average center-to-center distance of less than about 500 nm;
- and
- introducing said plurality of analytes to a solution comprising erythorbic acid.
Dependencies are inferred from the claim text.