DNA polymerase variant with improved discrimination of genetic variants

Abstract

The present invention relates to a DNA polymerase variant that belongs to family A, and to a use thereof. The present invention relates to: a DNA polymerase variant which easily undergoes polymerization when the base at the 3′-end of a primer is complementary to a template, and yet has inhibited polymerization when the base at the 3′-end of the primer is non-complementary to the template, and thus facilitates discrimination between the two cases; a PCR process using the variant; and a PCR kit comprising the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping), somatic mutation detection, and the like.

Dependencies are inferred from the claim text.