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US 12,747,464 B2Granted
Method for the biosynthesis of diosmin and/or hesperidin in a microorganism
AssigneeLes Laboratoires Servier
InventorsCyrille Pauthenier, André Le Jeune, Hélène Scornec, Célia Roussel, Laetitia Joubert
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Abstract
The present invention relates to a recombinant microorganism which is modified to be capable of producing diosmin and hesperidin and to the use thereof for producing diosmin and/or hesperidin.
Claims20
A method for producing diosmin and/or hesperidin, comprising culturing a recombinant microorganism in a culture medium and harvesting diosmin and/or hesperidin produced by said recombinant microorganism, wherein the recombinant microorganism comprises
- a heterologous nucleic acid sequence coding for a flavanone 7-O-beta-D-glucosyltransferase (UGT) belonging to EC 2.4.1.185, which is capable of adding a glucose in position 7 of hesperetin and/or diosmetin;
- and
- a heterologous nucleic acid sequence coding for a 6″-O-rhamnosyltransferase (RhaT) belonging to EC 2.4.1.B53, which is capable of transferring a rhamnose into position 6 of the glucose of hesperetin-7-O-glucoside and/or diosmetin-7-O-glucoside;
- and
- a heterologous nucleic acid sequence coding for a UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase (RHM) belonging to EC 4.2.1.76, which is capable of producing UDP-rhamnose.
2 more elements
Dependencies are inferred from the claim text.