Bacillus licheniformis cell stably and repeatedly used for conversion and synthesis of D-psicose

Abstract

The present disclosure discloses a Bacillus licheniformis cell stably and repeatedly used for the conversion and synthesis of D-psicose, and belongs to the field of biotechnology. D-psicose is a functional sugar with high health value and has broad application prospects in food, medicine, health care and other fields. The B. licheniformis cell of the present disclosure efficiently expresses recombinant D-psicose-3-epimerase, and can convert high-concentration fructose as a substrate into D-psicose. After the whole-cell catalyst is reused 10 times, the conversion rate is not less than 30%. Compared with pure enzyme or single-use whole-cell conversion methods, this technology for the synthesis of D-psicose has significant advantages such as low catalyst cost and simple separation and purification.

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