Back to results
US 12,747,457 B2Granted
Compositions comprising a CAS12I polypeptide and uses thereof
AssigneeArbor Biotechnologies, Inc.
InventorsNoah Michael Jakimo, Pratyusha Hunnewell, Brendan Jay Hilbert, David A. Scott
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions comprising a Cas12i polypeptide, a deaminase polypeptide, and an RNA guide, processes for characterizing the compositions, cells comprising the compositions, Cas12i fusion proteins, Cas12i complexes, and methods of using the compositions.
Claims42
A Cas12i fusion protein comprising:
- i) a Cas12i polypeptide comprising an alteration relative to the amino acid sequence of SEQ ID NO:
- 2, wherein the alteration is selected from the group comprising D581R, D911R, V1030G, S1046G, G624R, F626R, E1035R, and P868T, wherein the Cas12i polypeptide comprises at least 95% identity to the amino acid sequence of SEQ ID NO:
- 2, and wherein the Cas12i polypeptide has reduced nuclease activity or is a nuclease dead Cas12i polypeptide;
- and
- ii) a heterologous sequence comprising a deaminase domain.
2 more elements
Dependencies are inferred from the claim text.