Herbicide-tolerant genes and method for using same

A polypeptide, of which the amino acid sequence having the following mutation compared with the amino acid sequence of RdpA as set forth in SEQ ID NO:

• 1:
• the amino acid at position 82 is mutated from leucine into histidine;
• optionally, also having one or more mutation(s) selected from the following groups:
• the amino acid at position 187 is mutated from valine into leucine, methionine or isoleucine;
• the amino acid at position 187 is mutated from valine into leucine, and the amino acid at position 104 is mutated from arginine into alanine, aspartic acid or leucine;
• the amino acid at position 187 is mutated from valine into leucine, and the amino acid at position 182 is mutated from phenylalanine into tryptophan;
• the amino acid at position 187 is mutated from valine into leucine, and the amino acid at position 103 is mutated from glycine into leucine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, and the amino acid at position 104 is mutated from arginine into glycine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, and the amino acid at position 103 is mutated from glycine into leucine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 112 is mutated from threonine into serine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 80 is mutated from valine into threonine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 180 is mutated from arginine into tryptophan or methionine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 108 is mutated from aspartic acid into cysteine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 109 is mutated from aspartic acid into glutamic acid;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 219 is mutated from glutamine into cysteine or proline;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 103 is mutated from glycine into leucine, and the amino acid at position 180 is mutated from arginine into aspartic acid, glutamic acid, serine, leucine, tryptophan or threonine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 103 is mutated from glycine into leucine, and the amino acid at position 80 is mutated from valine into threonine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 103 is mutated from glycine into leucine, and the amino acid at position 112 is mutated from threonine into alanine, serine or methionine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 103 is mutated from glycine into leucine, and the amino acid at position 247 is mutated from phenylalanine into tyrosine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 77 is mutated from valine into isoleucine;
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 104 is mutated from arginine into glycine, the amino acid at position 112 is mutated from threonine into serine, and the amino acid at position 180 is mutated from arginine into lysine, methionine, tryptophan or glutamine;
• and/or
• the amino acid at position 187 is mutated from valine into leucine, the amino acid at position 182 is mutated from phenylalanine into tryptophan, the amino acid at position 103 is mutated from glycine into leucine, the amino acid at position 104 is mutated from arginine into glycine, and the amino acid at position 105 is mutated from valine into tyrosine;
• wherein, the amino acid sequence of the polypeptide further has at least 80% sequence identity to the amino acid sequence of RdpA as set forth in SEQ ID NO:
• 1.