Transformed plant having blue flower color, and method for creating same

A transformed rose plant or an inbred or an outbred progeny thereof, a propagule thereof, a part thereof, a tissue or a cell of the transformed rose plant or the inbred or the outbred progeny thereof, comprising:

• (a) a flavone synthase (FNS) gene encoding an FNS having at least 90% sequence identity to SEQ ID NO:
• 20, a first flavone C-glucosyltransferase (CGT) gene encoding a CGT having at least 90% sequence identity to SEQ ID NO:
• 22, wherein an Arabidopsis alcohol dehydrogenase (ADH) gene-derived 5′ untranslated region (5′-UTR) (SEQ ID NO:
• 23) has been operably linked to the 5′ end of the CGT gene, a flavonoid 3′,5′-hydroxylase (F3′5′H) gene encoding an F3′5′H having at least 90% sequence identity to SEQ ID NO:
• 10, and a methyltransferase (MT) gene encoding an MT having at least 90% sequence identity to SEQ ID NO:
• 18;
• or
• (b) a flavanone 2-hydroxylase (F2H) gene encoding an F2H having at least 90% sequence identity to SEQ ID NO:
• 4, a second flavone C-glucosyltransferase (CGT) gene encoding a CGT having at least 90% sequence identity to SEQ ID NO:
• 14, a dehydratase (FDH) gene encoding a FDH having at least 90% sequence identity to SEQ ID NO:
• 16, a flavonoid 3′,5′-hydroxylase (F3′5′H) gene encoding an F3′5′H having at least 90% sequence identity to SEQ ID NO:
• 10, and a methyltransferase (MT) gene encoding an MT having at least 90% sequence identity to SEQ ID NO:
• 18,
• so that a delphinidin-type anthocyanin coexists with a flavone C-glycoside in a cell of the rose plant,
• wherein the flavone C-glycoside is selected from the group consisting of flavone 6-C-glucoside, flavone 8-C glucoside, and a combination thereof,
• wherein the transformed rose plant is obtained from a rose of Ocean Song variety, and
• wherein the transformed rose plant or inbred or outbred progeny thereof has a flower color belonging to the Blue group or Violet-Blue group of the RHS color chart and/or a hue angle of 339.7° to 270.0° in the CIE L*a*b* color system.

A vector comprising (a) a flavone synthase (FNS) gene encoding an FNS having at least 90% sequence identity to SEQ ID NO:

• 20;
• (b) a first flavone C-glucosyltransferase (CGT) gene (i) encoding a CGT having at least 90% sequence identity to SEQ ID NO:
• 22 and (ii) further comprising an operably linked Arabidopsis alcohol dehydrogenase (ADH) gene-derived 5′ untranslated region (5′-UTR) (SEQ ID NO:
• 23);
• (c) a flavonoid 3′,5′-hydroxylase (F3′5′H) gene encoding an F3′5′H having at least 90% sequence identity SEQ ID NO:
• 10;
• and (d) a methyltransferase (MT) gene encoding an MT having at least 90% sequence identity SEQ ID NO:
• 18.

A vector comprising (a) a flavanone 2-hydroxylase (F2H) gene encoding an F2H having at least 90% sequence identity to SEQ ID NO:

• 4;
• (b) a second flavone C-glucosyltransferase (CGT) gene encoding a CGT having at least 90% sequence identity to SEQ ID NO:
• 14;
• (c) a dehydratase (FDH) gene encoding an FDH having at least 90% sequence identity to SEQ ID NO:
• 16;
• (d) a flavonoid 3′,5′-hydroxylase (F3′5′H) gene encoding an F3′5′H having at least 90% sequence identity to SEQ ID NO:
• 10;
• and (e) a methyltransferase (MT) gene encoding an MT having at least 90% sequence identity to SEQ ID NO:
• 18.