Methods and compositions for increasing harvestable yield via editing GA20 oxidase genes to generate short stature plants
AssigneeMonsanto Technology LLC
InventorsSivalinganna Manjunath, Linda A. Rymarquis, Thomas L. Slewinski
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.
Claims25
A modified corn plant comprising a mutant allele of a GA20 oxidase_3gene at an endogenous GA20 oxidase_3 locus, wherein a wild-type allele of the GA20 oxidase_3gene encodes a GA20 oxidase_3 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 9, and a mutant allele of a GA20 oxidase_5 gene at an endogenous GA20 oxidase_5 locus, wherein a wild-type allele of the GA20 oxidase_5 gene encodes a GA20oxidase_5 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 15, wherein said modified corn plant is homozygous for the mutant allele of the GA20oxidase_3 gene and homozygous for the mutant allele of the GA20 oxidase_5 gene, wherein said modified corn plant:
- (a) has a 20% to 50% reduction in plant height relative to an unmodified control plant;
- and
- (b) has an ear with no significant off-types, wherein said ear has:
- (i) a number of kernels per ear that is at least 90% of the number of kernels per ear of the unmodified control plant;
- or
- (ii) an average single kernel weight per ear that is at least 90% of the average single kernel weight per ear of the unmodified control plant.
Dependencies are inferred from the claim text.