Method for production of Varicella Zoster Virus surface protein antigen

A method for producing Varicella Zoster Virus (VZV) surface protein (gE) antigens, comprising steps of:

• (a) culturing a recombinant cell line prepared using Chinese hamster ovary (CHO) cell that produces the VZV gE antigens, to obtain a culture solution;
• and
• (b) purifying the culture solution,
• wherein the culturing step (a) comprises steps of:
• (a-1) performing seed culture on the recombinant cell line;
• and
• (a-2) performing production culture on the cell line that has undergone the seed culture, wherein the step (a-2) comprises culturing the cell line at a temperature of 34° C. to 35.5° C., and
• wherein the purifying step (b) comprises steps of:
• (b-1) performing anion exchange chromatography;
• (b-2) performing hydrophobic interaction chromatography, comprising adding sodium chloride to an eluate obtained through the anion exchange chromatography and loading it onto a hydrophobic interaction chromatography column, and washing with a washing buffer containing sodium chloride;
• (b-3) subjecting the culture solution to treatment with a virus-inactivating agent, to inactivate viruses, and then performing concentration and diafiltration;
• (b-4) performing mixed-mode chromatography, to obtain an eluate;
• and
• (b-5) subjecting the eluate to concentration and diafiltration, and then performing nanofiltration, the steps being performed in a series of sequences.