Universal chimeric antigen expressing immune cells for targeting of diverse multiple antigens and method of manufacturing the same and use of the same for treatment of cancer, infections and autoimmune disorders

An isolated nucleic acid molecule encoding a polypeptide comprising from amino to carboxy terminus:

• a tag-binding domain, wherein the tag-binding domain is an antibody or antigen-binding fragment thereof comprising a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:
• 33 and a light chain variable domain comprising the amino acid sequence of SEQ ID NO:
• 34, that binds to a tag comprising the amino acid sequence of SEQ ID NO:
• 44,
• an extracellular hinge and transmembrane domain of CD28, and
• a cytoplasmic signal transduction domain of CD28,
• wherein the extracellular hinge and transmembrane domain of CD28 comprise amino acid sequences encoded by nucleotide sequences according to SEQ ID NOs:
• 6 and 7, respectively, and
• wherein the cytoplasmic signal transduction domain of CD28 comprises an amino acid sequence encoded by a nucleotide sequence according to SEQ ID NO:
• 8.