Fusion polypeptide and polypeptide dimer, and use thereof

Abstract

The present invention provides a fusion polypeptide, comprising a carrier protein and a polypeptide of interest, wherein the carrier protein has a plurality of coil domains linked by a ring, the polypeptide of interest is inserted in the ring of the carrier protein, and the carrier protein masks a site of interest on the polypeptide of interest, thereby blocking the accessibility of the site. The present invention also provides a polypeptide dimer, comprising a first polypeptide and a second polypeptide, wherein the first polypeptide comprises a first dimerization domain and a polypeptide of interest, the second polypeptide comprises a second dimerization domain and a binding domain, and the binding domain can bind to a site of interest on the polypeptide of interest. The present invention also provides a polynucleotide and a vector encoding the fusion polypeptide and polypeptide dimer, and a host cell comprising the polynucleotide and/or vector. Furthermore, the present invention also provides use of the fusion polypeptide and polypeptide dimer of the present invention in the treatment of cancer and/or the activation of immune cells.

Dependencies are inferred from the claim text.