Av3 mutant polypeptides for pest control
AssigneeSuterra, LLC
InventorsLin Bao, Breck Davis, Kyle Douglas Schneider
Life of this patent
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Abstract
New insecticidal peptides, polypeptides, proteins, and nucleotides; their expression in culture and plants; methods of producing the peptides, polypeptides, proteins, and nucleotides; new processes; new production techniques; new formulations; and new organisms, are disclosed. The present disclosure is also related to mutants named Av3 mutant polypeptides (AMPs) that are non-naturally occurring, modified-form of the peptide, Av3, isolated from the sea anemone, Anemonia viridis. Here we describe: polynucleotides encoding AMPs; various formulations and combinations of both polynucleotides and peptides; and methods for using the same that are useful for the control of insects.
Claims24
An Av3 mutant polypeptide (AMP) comprising or consisting of an amino acid sequence as set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40, or an agriculturally acceptable salt thereof.
A polynucleotide capable of encoding an AMP comprising or consisting of an amino acid sequence as set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40.
A method of producing an AMP, the method comprising:
- (a) preparing a vector comprising a first expression cassette comprising, or consisting of, a polynucleotide encoding an AMP, said AMP comprising an amino acid sequence of any one of SEQ ID NOs:
- 25, 36, 38, and 40;
- (b) introducing the vector into a host cell;
- and
- (c) growing the host cell in a growth medium under conditions allowing expression of the AMP and secretion into the growth medium.
A method of combating, controlling, or inhibiting a pest comprising, applying a pesticidally effective amount of:
- (1) an Av3 mutant polypeptide (AMP) comprising or consisting of an amino acid sequence set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40, or an agriculturally acceptable salt thereof;
- (2) a combination or mixture comprising two or more AMPs, each AMP comprising or consisting of an amino acid sequence set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40, or an agriculturally acceptable salt thereof, wherein the two or more AMPs are the same or different;
- or (3) a composition comprising one or more AMPs comprising or consisting of an amino acid sequence set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40, or an agriculturally acceptable salt thereof, to:
- the pest, a locus of the pest, a food supply of the pest, a habitat of the pest, or a breeding ground of the pest;
- a plant, a seed, a plant part, a locus of a plant, or an environment of a plant that is susceptible to an attack by the pest;
- an animal, a locus of an animal, or an environment of an animal susceptible to an attack by the pest;
- or a combination thereof.
A vector comprising a polynucleotide encoding an AMP having an amino acid sequence set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40.
A yeast strain comprising:
- a first expression cassette comprising a polynucleotide encoding an AMP, said AMP comprising an amino acid sequence as set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40.
An Av3 mutant polypeptide (AMP) having insecticidal activity against one or more insect species, said AMP comprising, consisting essentially of, or consisting of, an amino acid sequence set forth in any one of SEQ ID NOs:
- 25, 36, 38, and 40.
Dependencies are inferred from the claim text.