Leukocyte concentration separation device, blood collection container, and method for separating leukocytes

A blood collection container by which a predetermined amount of blood is collected, the blood collection container comprising:

• a blood collection container main body;
• and
• a hemocyte separation material contained in the blood collection container main body,
• the hemocyte separation material having a specific gravity at 25 °C of 1.082 or more and 1.090 or less, and
• the blood collection container including a configuration A2 and a configuration B2 below:
• a configuration A2:
• the blood collection container including a hemagglutinating agent contained in the blood collection container main body;
• a configuration B2:
• the blood collection container including an osmotic pressure regulator contained in the blood collection container main body, and when a water-soluble component contained in the blood collection container main body is dissolved with physiological saline in an amount equal to the predetermined amount of blood to be collected in the blood collection container to obtain a solution for osmotic pressure measurement, the solution for osmotic pressure measurement having an osmotic pressure of 320 mOsm/L or more and 360 mOsm/L or less.

A leukocyte concentration separation system in which a predetermined amount of a blood-derived sample is collected, the leukocyte concentration separation system being intended to concentrate and separate leukocytes present in the blood-derived sample, the leukocyte concentration separation system comprising a hemocyte separation material having a specific gravity at 25 °C of 1.082 or more and 1.090 or less, and the leukocyte concentration separation system including a configuration A3 and a configuration B3 below:

• a configuration A3:
• the leukocyte concentration separation system including a hemagglutinating agent;
• a configuration B3:
• the leukocyte concentration separation system including an osmotic pressure regulator, and when a water-soluble component to be mixed with the blood-derived sample is dissolved with physiological saline in an amount equal to the predetermined amount of the blood-derived sample to obtain a solution for osmotic pressure measurement, the solution for osmotic pressure measurement having an osmotic pressure of 320 mOsm/L or more and 360 mOsm/L or less.